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human kcnj13  (OriGene)


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    OriGene human kcnj13
    Human Kcnj13, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+kcnj13/Kir7%2E1+(KCNJ13)+(NM_002242)+Human+Untagged+Clone/pm23977131-73-20-33
    Average 90 stars, based on 1 article reviews
    human kcnj13 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Snowflake Vitreoretinal Degeneration (SVD) Mutation R162W Provides New Insights into Kir7.1 Ion Channel Structure and Function
    Article Snippet: .. To express the Kir7.1 channel and to study its electrophysiology, the full length cDNA clone of human KCNJ13 (NM_002242.2) cloned into the pCMV6-XL5 eukaryotic expression vector was obtained from ORIGENE (Cat no.TC128213, Rockville, MD). .. To generate an N-terminal Green Fluorescence Protein (GFP) construct, we amplified the KCNJ13 open reading frame flanked by the EcoR1 and BamH1 restriction sites using primer pairs: Forward-( 5′-GCTTCGAATTCCGACAGCAGTAATTGC-3′ ), and Reverse-( 5′-ATCCGGTGGATCCTTATTCTGTCAGTCC-3′ ).

    Article Title: Snowflake vitreoretinal degeneration (SVD) mutation R162W provides new insights into Kir7.1 ion channel structure and function.
    Article Snippet: .. Plasmid Vectors and Transfection To express the Kir7.1 channel and to study its electrophysiology, the full length cDNA clone of human KCNJ13 (NM_002242.2) cloned into the pCMV6-XL5 eukaryotic expression vector was obtained from ORIGENE (Cat no.TC128213, Rockville, MD). .. To generate an N-terminal Green Fluorescence Protein (GFP) construct, we amplified the KCNJ13 open reading frame flanked by the EcoR1 and BamH1 restriction sites using primer pairs: Forward-(59-GCTTCGAATTCCGACAGCAGTAATTGC-39), and Reverse-(59-ATCCGGTGGATCCTTATTCTGTCAGTCC39).

    Plasmid Preparation:

    Article Title: Snowflake vitreoretinal degeneration (SVD) mutation R162W provides new insights into Kir7.1 ion channel structure and function.
    Article Snippet: .. Plasmid Vectors and Transfection To express the Kir7.1 channel and to study its electrophysiology, the full length cDNA clone of human KCNJ13 (NM_002242.2) cloned into the pCMV6-XL5 eukaryotic expression vector was obtained from ORIGENE (Cat no.TC128213, Rockville, MD). .. To generate an N-terminal Green Fluorescence Protein (GFP) construct, we amplified the KCNJ13 open reading frame flanked by the EcoR1 and BamH1 restriction sites using primer pairs: Forward-(59-GCTTCGAATTCCGACAGCAGTAATTGC-39), and Reverse-(59-ATCCGGTGGATCCTTATTCTGTCAGTCC39).

    Transfection:

    Article Title: Snowflake vitreoretinal degeneration (SVD) mutation R162W provides new insights into Kir7.1 ion channel structure and function.
    Article Snippet: .. Plasmid Vectors and Transfection To express the Kir7.1 channel and to study its electrophysiology, the full length cDNA clone of human KCNJ13 (NM_002242.2) cloned into the pCMV6-XL5 eukaryotic expression vector was obtained from ORIGENE (Cat no.TC128213, Rockville, MD). .. To generate an N-terminal Green Fluorescence Protein (GFP) construct, we amplified the KCNJ13 open reading frame flanked by the EcoR1 and BamH1 restriction sites using primer pairs: Forward-(59-GCTTCGAATTCCGACAGCAGTAATTGC-39), and Reverse-(59-ATCCGGTGGATCCTTATTCTGTCAGTCC39).



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